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Bruce  Gale
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Phone: 801-585-5944
Office: 2122 MEB

Bruce Gale

Assistant Professor, Mechanical Engineering
Adjunct Professor, Electrical and Computer Engineering
Adjunct Professor, Bioengineering

Research

Biomedical Microsystems (BioMEMS), microfluidics, microarray fabrication, biosensors, DNA analysis, protein interactions


Current Research

Current research efforts are directed in these areas:
1. Printing of high-quality protein microarrays and associated highly parallel fluid handling systems
2. Lab-on-a-chip systems for diagnostic applications utilizing DNA analysis techniques
3. Nanoparticle, polymer, and small molecule analysis and separation using field flow fractionation
4. Integration of microfluidic components including: micropumps, microvalves, micromixers, sensors, sample preparation (DNA extraction), and biomolecule detectors/sensors
5. Manufacturing techniques for use in biomedical microfluidic systems


Selected Publications

Arellano JA, Howell TA, Gammon J, Cho S, Janát-Amsbury MM, Gale B, Use of a highly parallel microfluidic flow cell array to determine therapeutic drug dose response curves. Biomed Microdevices 2017 Jun;19(2):25

Labroo P, Shea J, Sant H, Gale B, Agarwal J, Effect Of combining FK506 and neurotrophins on neurite branching and elongation. Muscle Nerve 2017 Apr;55(4):570-581

Li H, Gale B, Shea J, Sant H, Terry CM, Agarwal J, Vascular Coupling System for End-to-End Anastomosis: An In Vivo Pilot Case Report. Cardiovasc Eng Technol 2017 Mar;8(1):91-95

Labroo P, Ho S, Sant H, Shea J, Gale BK, Agarwal J, Controlled Delivery of FK506 to Improve Nerve Regeneration. Shock 2016 Sep;46(3 Suppl 1):154-9

Ragsdale V, Li H, Sant H, Ameel T, Gale BK, A disposable, continuous-flow polymerase chain reaction device: design, fabrication and evaluation. Biomed Microdevices 2016 Aug;18(4):62

Samuel R, Badamjav O, Murphy KE, Patel DP, Son J, Gale BK, Carrell DT, Hotaling JM, Microfluidics: The future of microdissection TESE? Syst Biol Reprod Med 2016 Jun;62(3):161-70

Deng Y, Chen J, Zhao Y, Yan X, Zhang L, Choy K, Hu J, Sant HJ, Gale BK, Tang T, Transdermal Delivery of siRNA through Microneedle Array. Sci Rep 2016 Feb 18;6:21422

Ornthai M, Siripinyanond A, Gale BK, Effect of Ionic and Nonionic Carriers in Electrical Field-Flow Fractionation. Anal Chem 2016 Feb 2;88(3):1794-803

Ornthai M, Siripinyanond A, Gale BK, Biased cyclical electrical field-flow fractionation for separation of submicron particles. Anal Bioanal Chem 2016 Jan;408(3):855-63

Li H, Gale BK, Sant H, Shea J, David Bell E, Agarwal J, A Novel Vascular Coupling System for End-to-End Anastomosis. Cardiovasc Eng Technol 2015 Sep;6(3):294-302

Reid RC, Minteer SD, Gale BK, Contact lens biofuel cell tested in a synthetic tear solution. Biosens Bioelectron 2015 Jun 15;68:142-8

Jayamohan H, Gale BK, Minson B, Lambert CJ, Gordon N, Sant HJ, Highly sensitive bacteria quantification using immunomagnetic separation and electrochemical detection of guanine-labeled secondary beads. Sensors (Basel) 2015 May 22;15(5):12034-52

Samuel R, Stephenson R, Roy P, Pryor R, Zhou L, Bonkowsky JL, Gale BK, Microfluidic-aided genotyping of zebrafish in the first 48 h with 100% viability. Biomed Microdevices 2015 Apr;17(2):43

Davidoff SN, Au D, Gale BK, Brooks BD, Brooks AE, Maximizing Fibroblast Adhesion on Protein-Coated Surfaces Using Microfluidic Cell Printing. RSC Adv 2015 Jan 1;5(126):104101-104109

Petersen KE, Manangon E, Hood JL, Wickline SA, Fernandez DP, Johnson WP, Gale BK, A review of exosome separation techniques and characterization of B16-F10 mouse melanoma exosomes with AF4-UV-MALS-DLS-TEM. Anal Bioanal Chem 2014 Dec;406(30):7855-66

Tasci TO, Johnson WP, Fernandez DP, Manangon E, Gale BK, Circuit modification in electrical field flow fractionation systems generating higher resolution separation of nanoparticles. J Chromatogr A 2014 Oct 24;1365:164-72

Sundberg SO, Wittwer CT, Zhou L, Palais R, Dwight Z, Gale BK, Quasi-digital PCR: Enrichment and quantification of rare DNA variants. Biomed Microdevices 2014 Aug;16(4):639-44

Davidoff SN, Miles AR, Romanov V, Gale BK, Eckman JW, Brooks BD, The submerged printing of cells onto a modified surface using a continuous flow microspotter. J Vis Exp 2014 Apr 22;(86):

Gehrke C, Li H, Sant H, Gale B, Agarwal J, Design, fabrication and testing of a novel vascular coupling device. Biomed Microdevices 2014 Feb;16(1):173-80

Tasci TO, Johnson WP, Fernandez DP, Manangon E, Gale BK, Biased cyclical electrical field flow fractionation for separation of sub 50 nm particles. Anal Chem 2013 Dec 3;85(23):11225-32

Jayamohan H, Sant HJ, Gale BK, Applications of microfluidics for molecular diagnostics. Methods Mol Biol 2013;949:305-34

Gooch N, Burr RM, Holt DJ, Gale B, Ambati B, Design and in vitro biocompatibility of a novel ocular drug delivery device. J Funct Biomater 2013 Jan 18;4(1):14-26

Sant HJ, Chakravarty S, Merugu S, Ferguson CG, Gale BK, Characterization of polymerized liposomes using a combination of dc and cyclical electrical field-flow fractionation. Anal Chem 2012 Oct 2;84(19):8323-9

Merugu S, Sant HJ, Gale BK, Diffusion Split-Flow Thin Cell (SPLITT) system for protein separations. J Chromatogr B Analyt Technol Biomed Life Sci 2012 Aug 1;902:78-83

Merugu S, Sant HJ, Gale BK, A novel method for effective field measurements in electrical field-flow fractionation. Electrophoresis 2012 Mar;33(6):1040-7

Sant HJ, Gale BK, Characterization of a microscale thermal-electrical field-flow fractionation system. J Chromatogr A 2012 Feb 17;1225:174-81

Lin KM, Sant HJ, Gale BK, Agarwal JP, New approaches to bridge nerve gaps: development of a novel drug-delivering nerve conduit. Conf Proc IEEE Eng Med Biol Soc 2012;2012:747-50

Samuel R, Sant HJ, Jiao F, Johnson CR, Gale BK, Microfluidic laminate-based phantom for diffusion tensor-magnetic resonance imaging (DT-MRI). J Micromech Microeng 2011 Sep;21(9):950271-9502711

Jardine GJ, Wong GC, Elsnab JR, Gale BK, Ambati BK, Endocapsular carousel technique phacoemulsification. J Cataract Refract Surg 2011 Mar;37(3):433-7

Srinivas M, Sant HJ, Gale BK, Optimization of cyclical electrical field flow fractionation. Electrophoresis 2010 Oct;31(20):3372-9

Kim J, Miles A, Gale BK, Improved biomolecule microarrays by printing on nanoporous aluminum oxide using a continuous-flow microspotter. Small 2010 Jul 5;6(13):1415-21

Molokhia SA, Sant H, Simonis J, Bishop CJ, Burr RM, Gale BK, Ambati BK, The capsule drug device: novel approach for drug delivery to the eye. Vision Res 2010 Mar 31;50(7):680-5

Sundberg SO, Wittwer CT, Gao C, Gale BK, Spinning disk platform for microfluidic digital polymerase chain reaction. Anal Chem 2010 Feb 15;82(4):1546-50

Kim J, Johnson M, Hill P, Gale BK, Microfluidic sample preparation: cell lysis and nucleic acid purification. Integr Biol (Camb) 2009 Oct;1(10):574-86

Liu J, Eddings MA, Miles AR, Bukasov R, Gale BK, Shumaker-Parry JS, In situ microarray fabrication and analysis using a microfluidic flow cell array integrated with surface plasmon resonance microscopy. Anal Chem 2009 Jun 1;81(11):4296-301

Kim J, Surapaneni R, Gale BK, Rapid prototyping of microfluidic systems using a PDMS/polymer tape composite. Lab Chip 2009 May 7;9(9):1290-3

Sincic RS, Chang-Yen DA, Eddings M, Barrows LR, Gale BK, Parallel determination of phenotypic cytotoxicity with a micropattern of mutant cell lines. Biomed Microdevices 2009 Apr;11(2):443-52

Crews N, Wittwer CT, Montgomery J, Pryor R, Gale B, Spatial DNA melting analysis for genotyping and variant scanning. Anal Chem 2009 Mar 15;81(6):2053-8

Rich RL, Miles AR, Gale BK, Myszka DG, Detergent screening of a G-protein-coupled receptor using serial and array biosensor technologies. Anal Biochem 2009 Mar 1;386(1):98-104

Eddings MA, Eckman JW, Arana CA, Papalia GA, Connolly JE, Gale BK, Myszka DG, "Spot and hop": internal referencing for surface plasmon resonance imaging using a three-dimensional microfluidic flow cell array. Anal Biochem 2009 Feb 15;385(2):309-13

Eddings MA, Miles AR, Eckman JW, Kim J, Rich RL, Gale BK, Myszka DG, Improved continuous-flow print head for micro-array deposition. Anal Biochem 2008 Nov 1;382(1):55-9

Crews N, Ameel T, Wittwer C, Gale B, Flow-induced thermal effects on spatial DNA melting. Lab Chip 2008 Nov;8(11):1922-9

Natarajan S, Hatch A, Myszka DG, Gale BK, Optimal conditions for protein array deposition using continuous flow. Anal Chem 2008 Nov 15;80(22):8561-7

Smith KA, Gale BK, Conboy JC, Micropatterned fluid lipid bilayer arrays created using a continuous flow microspotter. Anal Chem 2008 Nov 1;80(21):7980-7

Kim J, Gale BK, Quantitative and qualitative analysis of a microfluidic DNA extraction system using a nanoporous AlO(x) membrane. Lab Chip 2008 Sep;8(9):1516-23

Crews N, Wittwer C, Palais R, Gale B, Product differentiation during continuous-flow thermal gradient PCR. Lab Chip 2008 Jun;8(6):919-24

Crews N, Wittwer C, Gale B, Continuous-flow thermal gradient PCR. Biomed Microdevices 2008 Apr;10(2):187-95

Natarajan S, Katsamba PS, Miles A, Eckman J, Papalia GA, Rich RL, Gale BK, Myszka DG, Continuous-flow microfluidic printing of proteins for array-based applications including surface plasmon resonance imaging. Anal Biochem 2008 Feb 1;373(1):141-6

Zhang F, Gates RJ, Smentkowski VS, Natarajan S, Gale BK, Watt RK, Asplund MC, Linford M , Direct Adsorption and Detection of Proteins, Including Ferritin, onto Microlens Array Patterned Bioarrays. J Am Chem Soc 2007 Jul 10;:

Sundberg SO, Wittwer CT, Greer J, Pryor RJ, Elenitoba-Johnson O, Gale BK, Solution-phase DNA mutation scanning and SNP genotyping by nanoliter melting analysis. Biomed Microdevices 2007 Apr;9(2):159-66

Sant HJ, Kim JW, Gale BK, Reduction of end effect-induced zone broadening in field-flow fractionation channels. Anal Chem 2006 Dec 1;78(23):7978-85

Kantak A, Merugu S, Gale BK, Improved theory of cyclical electrical field flow fractionation. Electrophoresis 2006 Jul;27(14):2833-43

Kantak A, Srinivas M, Gale B, Characterization of a microscale cyclical electrical field flow fractionation system. Lab Chip 2006 May;6(5):645-54

Kantak AS, Srinivas M, Gale BK, Effect of carrier ionic strength in microscale cyclical electrical field-flow fractionation. Anal Chem 2006 Apr 15;78(8):2557-64

Sant HJ, Gale BK, Geometric scaling effects on instrumental plate height in field flow fractionation. J Chromatogr A 2006 Feb 3;1104(1-2):282-90

Narayanan N, Saldanha A, Gale BK, A microfabricated electrical SPLITT system. Lab Chip 2006 Jan;6(1):105-14

Gale BK, Srinivas M, Cyclical electrical field flow fractionation. Electrophoresis 2005 May;26(9):1623-32

Chang-Yen DA, Gale BK, An integrated optical oxygen sensor fabricated using rapid-prototyping techniques. Lab Chip 2003 Nov;3(4):297-301

Gale BK, Caldwell KD, Frazier AB, Geometric scaling effects in electrical field flow fractionation. 2. Experimental results. Anal Chem 2002 Mar 1;74(5):1024-30

Gale BK, Caldwell KD, Frazier AB, A micromachined electrical field-flow fractionation (mu-EFFF) system. IEEE Trans Biomed Eng 1998 Dec;45(12):1459-69