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Vladimir  Hlady

Phone: 801-581-5042
Phone: 801-581-5188 (lab)
Office: 108A BPRB

Vladimir Hlady

Adjunct Professor, Departments of Medicinal Chemistry and Materials Science and Engineering
AIMBE College of Fellows
BS Chemistry, 1972, University of Zagreb
MS Chemistry, 1975, University of Zagreb
DSc Chemistry, 1981, University of Zagreb


Research in the Proteins - Polymers - Interfaces Group (PPIG) focuses on proteins and other macromolecules at biomaterial-host tissue interfaces.

Current Research

Recent research themes include:
- evaluating the role of upstream agonists on downstream platelets-biomaterial surface interactions,
- developing microfluidic assays for measuring the efficacy of anti-platelet agents,
- studying the role of astrocyte-expressed glycosaminoglycans in neuronal regeneration,
- measuring micromechanical properties of endothelial glycocalyx in lung microvasculature, and
- evaluating the role of biomaterial surface chemistry on the proteins-surface interactions and dynamics.

Our main approach is to observe interfacial events involving few molecules at short length and time scales. Surface Analysis and Imaging Laboratory utilizes various experimental techniques such as FCS, TIRF, SFM, QCM, SPR and others.

Most recent publication from Proteins - Polymers - Interfaces Group (PPIG) is:
Shekh M. Rahman, Colin D. Eichinger and Vladimir Hlady, Effects of Upstream Shear Forces on Priming Downstream Adhesion and Activation, Acta Biomaterialia 73 (2018) 228235; doi:j.actbio.2018.04.002

Selected Publications

Hsiao TW, Tresco PA, Hlady V, Astrocyte spreading and migration on aggrecan-laminin dot gradients. Biointerphases 2017 Sep 11;13(1):01A401

Eichinger CD, Hlady V, Binary agonist surface patterns prime platelets for downstream adhesion in flowing whole blood. Biointerphases 2017 Apr 28;12(2):02C406

Job KM, O'Callaghan R, Hlady V, Barabanova A, Dull RO, The Biomechanical Effects of Resuscitation Colloids on the Compromised Lung Endothelial Glycocalyx. Anesth Analg 2016 Aug;123(2):382-93

Eichinger CD, Fogelson AL, Hlady V, Functional assay of antiplatelet drugs based on margination of platelets in flowing blood. Biointerphases 2016 Jun 30;11(2):029805

Gooch NW, Hlady V, Two surface gradients of polyethylene glycol for a reduction in protein adsorption. Surf Innov 2015 Sep;3(3):172-180

Hsiao TW, Tresco PA, Hlady V, Astrocytes alignment and reactivity on collagen hydrogels patterned with ECM proteins. Biomaterials 2015 Jan;39:124-30

Hsiao TW, Swarup VP, Eichinger CD, Hlady V, Cell substrate patterning with glycosaminoglycans to study their biological roles in the central nervous system. Methods Mol Biol 2015;1229:457-67

Swarup VP, Hsiao TW, Zhang J, Prestwich GD, Kuberan B, Hlady V, Exploiting differential surface display of chondroitin sulfate variants for directing neuronal outgrowth. J Am Chem Soc 2013 Sep 11;135(36):13488-94

Hsiao TW, Swarup VP, Kuberan B, Tresco PA, Hlady V, Astrocytes specifically remove surface-adsorbed fibrinogen and locally express chondroitin sulfate proteoglycans. Acta Biomater 2013 Jul;9(7):7200-8

Swarup VP, Mencio CP, Hlady V, Kuberan B, Sugar glues for broken neurons. Biomol Concepts 2013 Jun;4(3):233-57

Hodgkinson GN, Tresco PA, Hlady V, The role of well-defined patterned substrata on the regeneration of DRG neuron pathfinding and integrin expression dynamics using chondroitin sulfate proteoglycans. Biomaterials 2012 Jun;33(17):4288-97

Job KM, Dull RO, Hlady V, Use of reflectance interference contrast microscopy to characterize the endothelial glycocalyx stiffness. Am J Physiol Lung Cell Mol Physiol 2012 Jun 15;302(12):L1242-9

Meng F, Hlady V, Tresco PA, Inducing alignment in astrocyte tissue constructs by surface ligands patterned on biomaterials. Biomaterials 2012 Feb;33(5):1323-35

Corum LE, Hlady V, The effect of upstream platelet-fibrinogen interactions on downstream adhesion and activation. Biomaterials 2012 Feb;33(5):1255-60

Eichinger CD, Hsiao TW, Hlady V, Multiprotein microcontact printing with micrometer resolution. Langmuir 2012 Jan 31;28(4):2238-43

Ding YX, Hlady V, Competitive Adsorption of Three Human Plasma Proteins onto Sulfhydryl-to-sulfonate Gradient Surfaces. Croat Chem Acta 2011 Oct;84(2):193-202

O'Callaghan R, Job KM, Dull RO, Hlady V, Stiffness and heterogeneity of the pulmonary endothelial glycocalyx measured by atomic force microscopy. Am J Physiol Lung Cell Mol Physiol 2011 Sep;301(3):L353-60

Corum LE, Eichinger CD, Hsiao TW, Hlady V, Using microcontact printing of fibrinogen to control surface-induced platelet adhesion and activation. Langmuir 2011 Jul 5;27(13):8316-22

Stewart RJ, Ransom TC, Hlady V, Natural Underwater Adhesives. J Polym Sci B Polym Phys 2011 Jun;49(11):757-771

Deshmukh V, Britt DW, Hlady V, Excess fibrinogen adsorption to monolayers of mixed lipids. Colloids Surf B Biointerfaces 2010 Dec 1;81(2):607-13

Ding YX, Streitmatter S, Wright BE, Hlady V, Spatial variation of the charge and sulfur oxidation state in a surface gradient affects plasma protein adsorption. Langmuir 2010 Jul 20;26(14):12140-6

Corum LE, Hlady V, Screening platelet-surface interactions using negative surface charge gradients. Biomaterials 2010 Apr;31(12):3148-55

Miller C, Komunjer L, Hlady V, Heterogeneous Nucleation of Dicalcium Phosphate Dihydrate on Modified Silica Surfaces. Med Vjesn (Osijek) 2010;42(3-4):63-72

Maran U, Britt D, Fox CB, Harris JM, Orendt AM, Conley H, Davis R, Hlady V, Stang PJ, Self-assembly of a triangle-shaped, hexaplatinum-incorporated, supramolecular amphiphile in solution and at interfaces. Chemistry 2009 Aug 24;15(34):8566-77

Hodgkinson GN, Tresco PA, Hlady V, The influence of sub-micron inhibitory clusters on growth cone substratum attachments and CD44 expression. Biomaterials 2008 Nov;29(31):4227-35

Maran U, Conley H, Frank M, Arif AM, Orendt AM, Britt D, Hlady V, Davis R, Stang PJ, Giant micelles of organoplatinum(II) gemini amphiphiles. Langmuir 2008 May 20;24(10):5400-10

Hodgkinson G, Hlady V, Relating material surface heterogeneity to protein adsorption: the effect of annealing of micro-contact-printed OTS patterns. J Adhes Sci Technol 2008;19(3-5):235-255

Hlady V, Hodgkinson G, The effects of proteoglycan surface patterning on neuronal pathfinding. Materwiss Werksttech 2007 Dec 1;38(12):975

Hodgkinson GN, Hlady V, How Surface Heterogeneity Affects Protein Adsorption: Annealing of OTS Patterns and Albumin Adsorption Kinetics. Croat Chem Acta 2007 Nov 1;80(3-4):405-420

Turner NW, Liu X, Piletsky SA, Hlady V, Britt DW, Recognition of conformational changes in beta-lactoglobulin by molecularly imprinted thin films. Biomacromolecules 2007 Sep;8(9):2781-7

Stevens AP, Hlady V, Dull RO, Fluorescence correlation spectroscopy can probe albumin dynamics inside lung endothelial glycocalyx. Am J Physiol Lung Cell Mol Physiol 2007 Aug;293(2):L328-35

Hodgkinson GN, Tresco PA, Hlady V, The differential influence of colocalized and segregated dual protein signals on neurite outgrowth on surfaces. Biomaterials 2007 Jun;28(16):2590-602

Stevens MJ, Steren RE, Hlady V, Stewart RJ, Multiscale structure of the underwater adhesive of Phragmatopoma californica: a nanostructured latex with a steep microporosity gradient. Langmuir 2007 Apr 24;23(9):5045-9

Turner NW, Wright BE, Hlady V, Britt DW, Formation of protein molecular imprints within Langmuir monolayers: a quartz crystal microbalance study. J Colloid Interface Sci 2007 Apr 1;308(1):71-80

Hlady V, Jogikalmath G, Albumin binding and insertion into PS-b-PEO monolayers at air-water interface. Colloids Surf B Biointerfaces 2007 Feb 15;54(2):179-87

Turner NW, Jeans CW, Brain KR, Allender CJ, Hlady V, Britt DW, From 3D to 2D: a review of the molecular imprinting of proteins. Biotechnol Prog 2006 Nov-Dec;22(6):1474-89

Du X, Hlady V, Britt D, Langmuir monolayer approaches to protein recognition through molecular imprinting. Biosens Bioelectron 2005 Apr 15;20(10):2053-60